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Proteintech gfp
Gfp, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp/GFP+tag+Antibody/pm42910938-260-70-72
Average 96 stars, based on 1660 article reviews
gfp - by Bioz Stars, 2026-11
96/100 stars

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Western Blot:

Article Title: OsCPN10a, cooperating with OsCPN20 and OsHSP60-3B negatively regulate ABA signaling and enhance seed storability in rice.
Article Snippet: Protein samples were then incubated with Anti‐GFP beads (Lablead, China, GNA‐20‐400) at 4°C for 2 h with gentle agitation. .. Immunoblotting was performed using anti‐GFP (66002‐ 1‐Ig, Proteintech, China, 1:5,000 dilution), anti‐MYC (10828‐ 1‐AP, Proteintech, China, 1:5,000 dilution), and anti‐HA (51064‐2‐AP, Proteintech, China, 1:5,000 dilution), along with anti‐mouse secondary antibody (CWBIO, CW0102S, 1:10,000 dilution). ..

Article Title: OsCPN10a, cooperating with OsCPN20 and OsHSP60-3B negatively regulate ABA signaling and enhance seed storability in rice.
Article Snippet: .. WB analysis was performed with a monoclonal mouse anti‐GFP (66002‐1‐Ig, Proteintech, China, 1:5,000 dilution) or anti‐GST (M20007S, Abmart, China, 1:5,000 dilution) antibodies, along with an anti‐mouse secondary antibody (CWBIO, CW0102S, 1:10,000 dilution). ..

Article Title: Differential targeting of human pyroptotic caspase-5 and caspase-4 by Shigella OspC2 and OspC3
Article Snippet: Anti-Flag M2 magnetic beads (M8823 Millipore Sigma), pre-washed with IP buffer (50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 5 mM MgCl 2 , 2 mM EDTA, 20 μg/mL Digitonin), were added to 1-2 mg/ml soluble fraction and incubated for 2 h at 4°C with constant rotation. .. Samples were loaded onto a 12% SDS-PAGE gel, followed by immunoblotting with anti-Flag, anti-GFP (66002-1, Proteintech), and anti-ADP-ribose antibodies, using conditions described above. .. Samples were loaded onto a 12% SDS-PAGE gel, followed by immunoblotting with anti-Flag, anti-GFP (66002-1, Proteintech), and anti-ADP-ribose antibodies, using conditions described above.

Article Title: Phosphorylation of the plant photoreceptor UVR8 regulates its dimer/monomer status and enhances UV-B signaling across species.
Article Snippet: After electrophoresis, proteins were transferred to PVDF membranes according to the manufacturer’s instructions (Bio-Rad). .. For immunoblot analyses, anti-UVR8426–440 (Favory et al. 2009), anti-UVR8410–424(Kaiserli and Jenkins 2007), anti-SlUVR8415–429 (Liu et al. 2020), anti-CHS (PAB09104; Orizymes Biotechnologies), anti-COP1 (A18844; Abclonal), anti-RUP2 (Ren et al. 2019), anti-H3 (ab1791; Abcam), anti-Ugpase (AS05086; Agrisera), and anti-GAPDH (60004-1-PBS; Proteintech), anti-GFP (66002-1-Ig; Proteintech) were used as primary antibody. .. Anti-Rabbit (Abmart) and Anti-mouse (Abmart) secondary antibodies were used as required.

other:

Article Title: FoTO1 orchestrates Taxol biosynthesis through catalytic and non-catalytic mechanisms
Article Snippet: Protein lysates were resolved on a NuPAGE gel (Invitrogen) at 100V for 1.5 h. Proteins were then transferred onto a PVDF membrane using a Bio-Rad Trans-Blot Turbo Transfer System.

Article Title: The EHMT2-MBLAC2 axis suppresses ribosomal DNA transcription in response to nucleolar DNA damage
Article Snippet: HeLa, U2OS, GES1 and HEK293T cells were obtained from ATCC and SW480 cells were obtained from Servicebio.

Article Title: Isoginkgetin protects against degeneration of ALS motor neurons via regulating the GSK-3β-TFEB signaling axis.
Article Snippet: Primary antibodies used in this study were as follows: anti-TFEB (37785, 1:1000; 91767, 1:100; Cell Signaling Technology), antiLAMP2 (34141, 1:1000; Cell Signaling Technology), anti-cathepsin B (31718, 1:1000; Cell Signaling Technology), anti-cathepsin D (88239.

SDS Page:

Article Title: Differential targeting of human pyroptotic caspase-5 and caspase-4 by Shigella OspC2 and OspC3
Article Snippet: Anti-Flag M2 magnetic beads (M8823 Millipore Sigma), pre-washed with IP buffer (50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 5 mM MgCl 2 , 2 mM EDTA, 20 μg/mL Digitonin), were added to 1-2 mg/ml soluble fraction and incubated for 2 h at 4°C with constant rotation. .. Samples were loaded onto a 12% SDS-PAGE gel, followed by immunoblotting with anti-Flag, anti-GFP (66002-1, Proteintech), and anti-ADP-ribose antibodies, using conditions described above. .. Samples were loaded onto a 12% SDS-PAGE gel, followed by immunoblotting with anti-Flag, anti-GFP (66002-1, Proteintech), and anti-ADP-ribose antibodies, using conditions described above.



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The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed <t>GFP-tagged</t> domains (green) in BmN4 cells. Representative merged bright-field and <t>GFP</t> fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with <t>an</t> <t>anti-GFP</t> antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.
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The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed <t>GFP-tagged</t> domains (green) in BmN4 cells. Representative merged bright-field and <t>GFP</t> fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with <t>an</t> <t>anti-GFP</t> antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.
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The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed <t>GFP-tagged</t> domains (green) in BmN4 cells. Representative merged bright-field and <t>GFP</t> fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with <t>an</t> <t>anti-GFP</t> antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.
Anti Gfp Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed <t>GFP-tagged</t> domains (green) in BmN4 cells. Representative merged bright-field and <t>GFP</t> fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with <t>an</t> <t>anti-GFP</t> antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.
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The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed <t>GFP-tagged</t> domains (green) in BmN4 cells. Representative merged bright-field and <t>GFP</t> fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with <t>an</t> <t>anti-GFP</t> antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.
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The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed <t>GFP-tagged</t> domains (green) in BmN4 cells. Representative merged bright-field and <t>GFP</t> fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with <t>an</t> <t>anti-GFP</t> antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.
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The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed <t>GFP-tagged</t> domains (green) in BmN4 cells. Representative merged bright-field and <t>GFP</t> fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with <t>an</t> <t>anti-GFP</t> antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.
Mouse Monoclonal Anti Gfp Clone B2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed GFP-tagged domains (green) in BmN4 cells. Representative merged bright-field and GFP fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with an anti-GFP antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.

Journal: iScience

Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

doi: 10.1016/j.isci.2026.117149

Figure Lengend Snippet: The BmMKLP1 domains exhibit divergent nuclear and cytoplasmic localization (A) Subcellular localization of transiently expressed GFP-tagged domains (green) in BmN4 cells. Representative merged bright-field and GFP fluorescence images acquired at 10×, scale bars, 100 μm, BmN4 cells expressing the indicated constructs were imaged by differential interference contrast (DIC) microscopy (left) to show cell morphology and by fluorescence microscopy (right) to detect GFP-tagged proteins. All images were acquired with a 40× objective lens. Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with an anti-GFP antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight. The “control” lane corresponds to the cycle 3 GFP-bleo fusion protein (∼39–40 KDa) expressed from the pIZT/V5-His vector. A representative blot from three independent experiments is shown. The PageRuler Prestained Protein Ladder (10–180 kDa; Thermo Fisher Scientific, Waltham, MA, USA) was used as a molecular weight marker.

Article Snippet: Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with an anti-GFP antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight.

Techniques: Fluorescence, Expressing, Construct, Microscopy, Morphology, Western Blot, Analysis, Transfection, Molecular Weight, Control, Plasmid Preparation, Marker

The head and stalk domains induce G2/M arrest, whereas the tail promotes polyploidization (A) Representative flow cytometry histograms showing DNA content profiles (top) and polyploidy distributions (bottom) for control cells and cells expressing GFP-tagged BmMKLP1—full-length, head, -stalk, or -tail. Cell cycle phases (G0/G1, S, and G2/M) were determined by model-based fitting of propidium iodide (PI) fluorescence intensity histograms. Polyploidy histograms depict cells with DNA content exceeding 4 N. (B) Quantification of cell cycle distribution under each condition. Bars represent the mean ± SEM percentage of cells in G0/G1, S, and G2/M phases from at least three independent experiments. Different lowercase letters indicate significant differences (one-way ANOVA followed by Tukey’s HSD, p < 0.05). (C) Percentage of polyploid cells under each condition. Bars represent mean ± SEM, with statistical significance assessed as in (B).

Journal: iScience

Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

doi: 10.1016/j.isci.2026.117149

Figure Lengend Snippet: The head and stalk domains induce G2/M arrest, whereas the tail promotes polyploidization (A) Representative flow cytometry histograms showing DNA content profiles (top) and polyploidy distributions (bottom) for control cells and cells expressing GFP-tagged BmMKLP1—full-length, head, -stalk, or -tail. Cell cycle phases (G0/G1, S, and G2/M) were determined by model-based fitting of propidium iodide (PI) fluorescence intensity histograms. Polyploidy histograms depict cells with DNA content exceeding 4 N. (B) Quantification of cell cycle distribution under each condition. Bars represent the mean ± SEM percentage of cells in G0/G1, S, and G2/M phases from at least three independent experiments. Different lowercase letters indicate significant differences (one-way ANOVA followed by Tukey’s HSD, p < 0.05). (C) Percentage of polyploid cells under each condition. Bars represent mean ± SEM, with statistical significance assessed as in (B).

Article Snippet: Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with an anti-GFP antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight.

Techniques: Flow Cytometry, Control, Expressing, Fluorescence

Journal: iScience

Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

doi: 10.1016/j.isci.2026.117149

Figure Lengend Snippet:

Article Snippet: Scale bars, 20 μm. (B) Western blot analysis of protein extracts from transfected BmN4 cells probed with an anti-GFP antibody (1:1,000, Proteintech, Wuhan, China), confirming the production of each GFP-tagged domain at its expected molecular weight.

Techniques: Transfection, Cloning, Imaging, Bicinchoninic Acid Protein Assay, Western Blot, Plasmid Preparation, Recombinant, Software