gfp (Proteintech)
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Gfp, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp/GFP+tag+Antibody/pm42910938-260-70-72
Average 96 stars, based on 1660 article reviews
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Western Blot:Article Title: OsCPN10a, cooperating with OsCPN20 and OsHSP60-3B negatively regulate ABA signaling and enhance seed storability in rice. Article Snippet: Protein samples were then incubated with Anti‐GFP beads (Lablead, China, GNA‐20‐400) at 4°C for 2 h with gentle agitation. .. Immunoblotting was performed using Article Title: OsCPN10a, cooperating with OsCPN20 and OsHSP60-3B negatively regulate ABA signaling and enhance seed storability in rice. Article Snippet: .. WB analysis was performed with a monoclonal mouse anti‐GFP (66002‐1‐Ig, Proteintech, China, 1:5,000 dilution) or anti‐GST (M20007S, Abmart, China, 1:5,000 dilution) antibodies, along with an anti‐mouse secondary antibody (CWBIO, CW0102S, 1:10,000 dilution). .. Article Title: Differential targeting of human pyroptotic caspase-5 and caspase-4 by Shigella OspC2 and OspC3 Article Snippet: Anti-Flag M2 magnetic beads (M8823 Millipore Sigma), pre-washed with IP buffer (50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 5 mM MgCl 2 , 2 mM EDTA, 20 μg/mL Digitonin), were added to 1-2 mg/ml soluble fraction and incubated for 2 h at 4°C with constant rotation. .. Samples were loaded onto a 12% SDS-PAGE gel, followed by immunoblotting with anti-Flag, Article Title: Phosphorylation of the plant photoreceptor UVR8 regulates its dimer/monomer status and enhances UV-B signaling across species. Article Snippet: After electrophoresis, proteins were transferred to PVDF membranes according to the manufacturer’s instructions (Bio-Rad). .. For immunoblot analyses, anti-UVR8426–440 (Favory et al. 2009), anti-UVR8410–424(Kaiserli and Jenkins 2007), anti-SlUVR8415–429 (Liu et al. 2020), anti-CHS (PAB09104; Orizymes Biotechnologies), anti-COP1 (A18844; Abclonal), anti-RUP2 (Ren et al. 2019), anti-H3 (ab1791; Abcam), anti-Ugpase (AS05086; Agrisera), and anti-GAPDH (60004-1-PBS; Proteintech), other:Article Title: FoTO1 orchestrates Taxol biosynthesis through catalytic and non-catalytic mechanisms Article Snippet: Protein lysates were resolved on a NuPAGE gel (Invitrogen) at 100V for 1.5 h. Proteins were then transferred onto a PVDF membrane using a Bio-Rad Trans-Blot Turbo Transfer System. Article Title: The EHMT2-MBLAC2 axis suppresses ribosomal DNA transcription in response to nucleolar DNA damage Article Snippet: HeLa, U2OS, GES1 and HEK293T cells were obtained from ATCC and SW480 cells were obtained from Servicebio. Article Title: Isoginkgetin protects against degeneration of ALS motor neurons via regulating the GSK-3β-TFEB signaling axis. Article Snippet: Primary antibodies used in this study were as follows: anti-TFEB (37785, 1:1000; 91767, 1:100; Cell Signaling Technology), antiLAMP2 (34141, 1:1000; Cell Signaling Technology), anti-cathepsin B (31718, 1:1000; Cell Signaling Technology), anti-cathepsin D (88239. SDS Page:Article Title: Differential targeting of human pyroptotic caspase-5 and caspase-4 by Shigella OspC2 and OspC3 Article Snippet: Anti-Flag M2 magnetic beads (M8823 Millipore Sigma), pre-washed with IP buffer (50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 5 mM MgCl 2 , 2 mM EDTA, 20 μg/mL Digitonin), were added to 1-2 mg/ml soluble fraction and incubated for 2 h at 4°C with constant rotation. .. Samples were loaded onto a 12% SDS-PAGE gel, followed by immunoblotting with anti-Flag, |
